손바닥 선인장 열매 추출물의 Streptococcus mutans에 대한 성장 억제 효과
In vitro growth inhibition of Streptococcus mutans by extract of prickly pear (Opuntia ficus-indica var. saboten Makino)
정은주, 홍석진, 정성숙, 최충호, 최정이, 오한나, 이혜진,
소속 상세정보
정은주 ( Jung Eun-Ju ) - 전남대학교 치의학전문대학원 예방치과학교실
홍석진 ( Hong Suk-Jin ) - 전남대학교 치의학전문대학원 예방치과학교실
정성숙 ( Jeong Seong-Soog ) - 전남대학교 치의학전문대학원 예방치과학교실
최충호 ( Choi Choong-Ho ) - 전남대학교 치의학전문대학원 BK21사업단
최정이 ( Choi Jeong Iee ) - 아주대학교 의과대학 생리학교실
오한나 ( Oh Han-Na ) - 전남대학교 치의학전문대학원 예방치과학교실
이혜진 ( Lee Hye-Jin ) - 전남대학교 치의학전문대학원 예방치과학교실
KMID : 0355420100340010028
Abstract
Objectives: Antimicrobial activity of extract of prickly pear (Opuntia ficus-indica var. saboten Makino; OFI) has been reported. This study examined effects of OFI extract on the growth of Streptococcus mutans.
Methods: OFI was extracted by 85% methanol using an ultrasonic extraction method. The agar plate dilution method was used to count the CFU of viability of S. mutans KCTC 3065 that was determined by enumeration (colony forming units) in an agar dilution assay using no OFI (control), or 5, 20, and 50 mg/mL OFI following incubation for 0, 6, 12, and 24 h. The cytotoxicity of OFI extract (0, 5, 10, 20, 40, 50 mg/mL) was evaluated for human gingival fibroblasts by a formazan-based viability.
Results: S. mutans growth was reduced by the OFI extract. The number of viable S. mutans was reduced significantly with increasing OFI concentration and incubation time (both p＜0.001). The viable counts of S. mutans cultured with 20 and 50 mg/mL OFI decreased gradually but appreciably (＞88.7%) by 6 h and no S. mutans remained when cultured with 50 mg/mL at 24 h. OFI concentrations of 40 and 50 mg/mL were cytotoxic to fibroblasts.
Conclusions: OFI displays S. mutans inhibitory activity, and may be potential exploited as an oral hygiene agent for the prevention of dental caries.
키워드
생약 추출물;성장 억제 효과;세포독성;손바닥 선인장 열매
cytotoxicity;growth inhibitory effect;microbiology;Opuntia ficus-indica var;Saboten Makino;Streptococcus mutans
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